HCV core protein-induced down-regulation of microRNA-152 promoted aberrant proliferation by regulating Wnt1 in HepG2 cells.

HCV core protein-induced down-regulation of microRNA-152 promoted aberrant proliferation by regulating Wnt1 in HepG2 cells.

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BackgroundHepatitis C virus (HCV) has been reported to regulate cellular microRNAs (miRNAs).The HCV core protein is considered to be a potential oncoprotein in HCV-related hepatocellular carcinoma (HCV-HCC), but HCV core-regulated miRNAs are largely unknown.Our preliminary experiments revealed significant down-regulation of microRNA-152 (miR-152) by HCV core protein in HepG2 cells.

Through target gene prediction softwares, Wnt1 was predicted to be a potential target of miR-152.The present study was initiated to investigate whether miR-152 hp victus 15l ryzen 5 is aberrantly regulated by the HCV core protein, and involved in the regulation of the aberrant proliferation of HCV-HCC cells.MethodsMiR-152 levels were examined by stem-loop real-time RT-PCR (SLqRT-PCR).

Cell proliferation was analyzed by MTT and colony formation assay.Cell cycle analysis was performed by flow cytometry.Luciferase reporter assay was conducted to confirm miRNA-target association.

Wnt1 expression was determined by real-time qPCR and Western blotting.ResultsHCV core protein significantly suppressed miR-152 expression, and led to significant Wnt1 up-regulation with a concomitant aberrantly promoted proliferation.Moreover, we validated that miR-152 inhibition promoted, while miR-152 mimics inhibited cell proliferation.

Using, qRT-PCR and western blot, Wnt1 was demonstrated to be regulated by miR-152.Luciferase activity assay showed that while miR-152 mimics significantly reduced the luciferase activity by 83.76% (PConclusionThese findings provide important evidence that the reduced miR-152 expression by HCV core protein quadruple ointment for dogs can indirectly lose an inhibitory effect on Wnt1, which might, at least partially lead to cell proliferation of liver cancer cells.

MiR-152 may have a therapeutic potential to suppress liver cancer proliferation.